Maintenance of High Cytochrome P450 Expression in HepaRG Cell Spheroids in DMSO-Free Medium

Bull Exp Biol Med. 2016 May;161(1):120-4. doi: 10.1007/s10517-016-3360-z. Epub 2016 Jun 6.


We studied the effects of DMSO and fibroblasts during HepaRG cell spheroid formation and conditions of their subsequent culturing on the levels of mRNA of the major cytochromes P450. A protocol of spheroid formation from differentiated HepaRG cells and their culturing in serum- and DMSO-free medium is developed.

Keywords: HepaRG; cytochromes P450; differentiation.

MeSH terms

  • Cell Line
  • Culture Media / chemistry
  • Cytochrome P-450 Enzyme System / genetics*
  • Cytochrome P-450 Enzyme System / metabolism
  • Dimethyl Sulfoxide / chemistry
  • Gene Expression
  • Gene Expression Regulation, Enzymologic
  • Hepatocytes / enzymology*
  • Humans
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Spheroids, Cellular / enzymology*


  • Culture Media
  • RNA, Messenger
  • Cytochrome P-450 Enzyme System
  • Dimethyl Sulfoxide