Dipeptide Piracetam Analogue Noopept Improves Viability of Hippocampal HT-22 Neurons in the Glutamate Toxicity Model

Bull Exp Biol Med. 2016 May;161(1):58-60. doi: 10.1007/s10517-016-3344-z. Epub 2016 Jun 6.


Effect of noopept (N-phenylacetyl-prolylglycine ethyl ester) on viability of neurons exposed to neurotoxic action of glutamic acid (5 mM) was studied in vitro in immortalized mouse hippocampal HT-22 neurons. Noopept added to the medium before or after glutamic acid improved neuronal survival in a concentration range of 10-11-10-5 M. Comparison of the effective noopept concentrations determined in previous studies on cultured cortical and cerebellar neurons showed that hippocampal neurons are more sensitive to the protective effect of noopept.

Keywords: HT-22 culture; dipeptides; glutamate toxicity; neuroprotection; noopept.

MeSH terms

  • Animals
  • Cell Line
  • Cell Survival / drug effects
  • Dipeptides / pharmacology*
  • Glutamic Acid / toxicity*
  • Mice
  • Neurons / cytology*
  • Neurons / drug effects*
  • Neuroprotective Agents / pharmacology*
  • Piracetam / analogs & derivatives*


  • Dipeptides
  • Neuroprotective Agents
  • Glutamic Acid
  • ethyl phenylacetyl-Pro-Gly
  • Piracetam