Blood biochemical and oxidant/antioxidant alterations following stanozolol treatment along with resistance training in rats

Andrologia. 2017 Mar;49(2). doi: 10.1111/and.12613. Epub 2016 Jun 8.


This study was designed to determine the effects of anabolic steroid stanozolol (ST) in conjunction with 8 weeks of resistance training on some blood biochemical and oxidant/antioxidant profile in rats. Twenty-four male rats were divided into four groups of six each: group 1: sedentary + placebo (physiological saline), group 2: training + placebo, group 3: training + ST (2 mg kg-1 b.w.) and group 4: training + ST (5 mg kg-1 b.w.). Erythrocytic activity of glutathione peroxidase was increased significantly in group 4 as compared to control group. Plasma activities of alanine aminotransferase in groups 3 and 4 and also aspartate aminotransferase in group 4 showed significant increase relative to groups 1 and 2. Moreover, alkaline phosphatase and creatine kinase activities increased significantly in groups 3 and 4 in comparison with control group. Elevated values of uric acid were significant in group 4, but not in groups 2 and 3, as compared to control group. Values of other measured parameters did not have significant alterations among experimental groups. Present findings indicate that stanozolol treatment in combination with resistance training induces some side effects especially on liver and muscle as evidenced by alterations in plasma markers of tissue damage.

Keywords: Biochemical markers; oxidative stress; resistance training; stanozolol.

MeSH terms

  • Alanine Transaminase / blood
  • Alkaline Phosphatase / blood
  • Anabolic Agents / administration & dosage
  • Anabolic Agents / adverse effects*
  • Animals
  • Aspartate Aminotransferases / blood
  • Creatine Kinase / blood
  • Glutathione Peroxidase / blood
  • Iran
  • Liver / drug effects*
  • Male
  • Muscles / drug effects
  • Oxidative Stress / drug effects*
  • Rats
  • Rats, Wistar
  • Resistance Training / adverse effects*
  • Stanozolol / administration & dosage
  • Stanozolol / adverse effects*
  • Uric Acid / blood


  • Anabolic Agents
  • Uric Acid
  • Stanozolol
  • Glutathione Peroxidase
  • Aspartate Aminotransferases
  • Alanine Transaminase
  • Creatine Kinase
  • Alkaline Phosphatase