Increased airway glucose increases airway bacterial load in hyperglycaemia

Sci Rep. 2016 Jun 8;6:27636. doi: 10.1038/srep27636.


Diabetes is associated with increased frequency of hospitalization due to bacterial lung infection. We hypothesize that increased airway glucose caused by hyperglycaemia leads to increased bacterial loads. In critical care patients, we observed that respiratory tract bacterial colonisation is significantly more likely when blood glucose is high. We engineered mutants in genes affecting glucose uptake and metabolism (oprB, gltK, gtrS and glk) in Pseudomonas aeruginosa, strain PAO1. These mutants displayed attenuated growth in minimal medium supplemented with glucose as the sole carbon source. The effect of glucose on growth in vivo was tested using streptozocin-induced, hyperglycaemic mice, which have significantly greater airway glucose. Bacterial burden in hyperglycaemic animals was greater than control animals when infected with wild type but not mutant PAO1. Metformin pre-treatment of hyperglycaemic animals reduced both airway glucose and bacterial load. These data support airway glucose as a critical determinant of increased bacterial load during diabetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Animals
  • Bacterial Infections / etiology*
  • Bacterial Infections / metabolism*
  • Bacterial Infections / microbiology
  • Bacterial Load
  • Blood Glucose
  • Critical Illness
  • Disease Models, Animal
  • Female
  • Gene Deletion
  • Genes, Bacterial
  • Glucose / metabolism*
  • Humans
  • Hyperglycemia / blood*
  • Hyperglycemia / complications*
  • Mice
  • Middle Aged
  • Mutation
  • Pseudomonas Infections / metabolism
  • Pseudomonas Infections / microbiology
  • Pseudomonas aeruginosa / physiology
  • Respiratory Mucosa / metabolism
  • Respiratory Mucosa / microbiology
  • Respiratory Tract Infections / etiology*
  • Respiratory Tract Infections / metabolism*
  • Respiratory Tract Infections / microbiology


  • Blood Glucose
  • Glucose