Reverse transcriptases lend a hand in splicing catalysis

Nat Struct Mol Biol. 2016 Jun 7;23(6):507-9. doi: 10.1038/nsmb.3242.

Abstract

The first high resolution structural views of group II intron maturases illuminate the architectural and functional roles of these multidomain proteins in splicing and DNA invasion. The maturases show striking structural and functional homology to a central protein involved in spliceosomal premessenger RNA splicing, solidifying the idea that group II introns and the spliceosome descended from a common ancestor, an ancient retroelement.

MeSH terms

  • Clostridiales / enzymology
  • Cryoelectron Microscopy
  • Crystallography, X-Ray
  • DNA / metabolism
  • Endoribonucleases / chemistry*
  • Endoribonucleases / metabolism*
  • Endoribonucleases / ultrastructure
  • Lactococcus lactis / enzymology
  • Models, Molecular
  • Nucleotidyltransferases / chemistry*
  • Nucleotidyltransferases / metabolism*
  • Nucleotidyltransferases / ultrastructure
  • Protein Conformation
  • RNA Splicing*
  • RNA-Directed DNA Polymerase / chemistry*
  • RNA-Directed DNA Polymerase / metabolism*
  • RNA-Directed DNA Polymerase / ultrastructure
  • Recombination, Genetic

Substances

  • DNA
  • Nucleotidyltransferases
  • mRNA maturase
  • RNA-Directed DNA Polymerase
  • Endoribonucleases