Continuous Enzymatic Biodiesel Production From Coconut Oil in Two-Stage Packed-Bed Reactor Incorporating an Extracting Column to Remove Glycerol Formed as By-Product

Bioprocess Biosyst Eng. 2016 Oct;39(10):1611-7. doi: 10.1007/s00449-016-1636-3. Epub 2016 Jun 8.


The transesterification of coconut oil with ethanol catalyzed by Burkholderia cepacia lipase immobilized on polysiloxane-polyvinyl alcohol was performed in a continuous flow. The experimental design consisted of a two-stage packed-bed reactor incorporating a column with cationic resin (Lewatit GF 202) to remove the glycerol formed as by-product and the reactor performance was quantified for three different flow rates corresponding to space-times from 10 to 14 h. The influence of space-time on the ethyl ester (FAEE) concentrations, yields and productivities was determined. The reactor operation was demonstrated for space-time of 14 h attaining FAEE concentrations of 58.5 ± 0.87 wt%, FAEE yields of 97.3 ± 1.9 % and productivities of 41.6 ± 1.0 mgester g medium (-1) h(-1). Biodiesel purified samples showed average kinematic viscosity values of 5.5 ± 0.3 mm(2) s(-1) that meet the criteria established by the American National Standard ASTM (D6751). The immobilized lipase was found to be stable regarding its morphological and catalytic characteristics, showing half-life time (t 1/2) around 1540 h. The continuous packed-bed reactor connected in series with simultaneous glycerol removal has a great potential to attain high level of transesterification yields, raising biodiesel productivity.

Keywords: Biodiesel; Coconut oil; Glycerol removal; Lipase; Packed-bed reactor.

MeSH terms

  • Biofuels*
  • Bioreactors*
  • Burkholderia cepacia / growth & development*
  • Coconut Oil
  • Glycerol / metabolism*
  • Plant Oils / metabolism*


  • Biofuels
  • Plant Oils
  • Glycerol
  • Coconut Oil