Evaluation of the topical spray containing Centella asiatica extract and its efficacy on excision wounds in rats

Acta Pharm. 2016 Jun 1;66(2):233-44. doi: 10.1515/acph-2016-0018.

Abstract

Centella asiatica was extracted by methanol. The assay content of triterpenes in the extract was 0.12 % asiatic acid, 0.54 % madecassic acid, 0.25 % asiaticoside and 1.02 % madecassoside. The extract was complexed with hydroxypropyl-β-cyclodextrin (HP-β-CD) and formulated with Eudragit E100, glycerol, PEG 400, copovidone, ethanol and purified water. A clear yellowish solution (F1-F8) was obtained. The formulations had a pH of 5.5-6.0 with viscosity in the range of 20-60 mPa s, surface tension 20.3-24.6 mN m-1 and contact angle less than 20°. The amount of PEG 400 and copovidone affected the film and spreadability. The content of triterpenes in the spray formulation was close to 100 % compared to triterpenes in the extract. The skin irritation study indicated that the formulation was non-irritating in a rat model. An in vivo excision wound healing model showed that wound excision was completely healed after 14 days.

MeSH terms

  • Administration, Cutaneous
  • Aerosols
  • Animals
  • Centella* / chemistry
  • Disease Models, Animal
  • Drug Compounding
  • Excipients / chemistry
  • Male
  • Phytotherapy
  • Plant Extracts / administration & dosage*
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification
  • Plants, Medicinal
  • Polyethylene Glycols / chemistry
  • Pyrrolidines / chemistry
  • Rats, Wistar
  • Surgical Wound / drug therapy*
  • Surgical Wound / pathology
  • Time Factors
  • Triterpenes / administration & dosage*
  • Triterpenes / chemistry
  • Triterpenes / isolation & purification
  • Vinyl Compounds / chemistry
  • Wound Healing / drug effects*

Substances

  • Aerosols
  • Centella asiatica extract
  • Excipients
  • Plant Extracts
  • Pyrrolidines
  • Triterpenes
  • Vinyl Compounds
  • poly(vinylpyrrolidone-co-vinyl-acetate)
  • Polyethylene Glycols
  • polyethylene glycol 400