Photoprotective Effect of Carpomitra costata Extract against Ultraviolet B-Induced Oxidative Damage in Human Keratinocytes

J Environ Pathol Toxicol Oncol. 2016;35(1):11-28. doi: 10.1615/JEnvironPatholToxicolOncol.2016014003.


Natural marine products show various biological properties such as antiphotoaging, antioxidant, anticancer, and anti-inflammation. This study evaluated the protective effects of the brown alga Carpomitra costata (Stackhouse) Batters (Sporochnaceae) against ultraviolet B (UVB)-provoked damage in human HaCaT keratinocytes. C. costata extract (CCE) effectively reduced superoxide anion, hydroxyl radical, and UVB-stimulated intracellular reactive oxygen species (ROS) levels. CCE also restored the expression and activity of UVB-suppressed antioxidant enzymes. Furthermore, CCE decreased UVB-triggered oxidative damage to cellular components including DNA, protein, and lipid and defended the cells against mitochondrial membrane depolarization-medicated apoptosis. The results of this study indicate that CCE can safeguard human keratinocytes against UVB-induced cellular damage via a potent antioxidant mechanism. CCE may find utility as part of a therapeutic arsenal against the damaging effects of UVB radiation on the skin.

MeSH terms

  • Antioxidants / metabolism*
  • Humans
  • Keratinocytes / drug effects*
  • Keratinocytes / radiation effects*
  • Phaeophyceae / chemistry*
  • Plant Extracts / pharmacology*
  • Skin Aging / drug effects*
  • Ultraviolet Rays*


  • Antioxidants
  • Plant Extracts