In Vivo Metal Ion Imaging Using Fluorescent Sensors

Genevieve C Van de Bittner; Tasuku Hirayama

doi:10.1007/978-1-4939-3721-9_13

In Vivo Metal Ion Imaging Using Fluorescent Sensors

Methods Mol Biol. 2016:1444:139-52. doi: 10.1007/978-1-4939-3721-9_13.

Authors

Genevieve C Van de Bittner¹, Tasuku Hirayama²

Affiliations

¹ Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA. gvandebittner@mgh.harvard.edu.
² Laboratory of Medicinal & Pharmaceutical Chemistry, Gifu Pharmaceutical University, Gifu, Japan.

PMID: 27283424
DOI: 10.1007/978-1-4939-3721-9_13

Abstract

In vivo imaging in living animals provides the ability to monitor alterations of signaling molecules, ions, and other biological components during various life stages and in disease. The data gained from in vivo imaging can be used for biological discovery or to determine elements of disease progression and can inform the development and translation of therapeutics. Herein, we present theories behind small-molecule, fluorescent, metal ion sensors as well as the methods for their successful application to in vivo metal ion imaging, including ex vivo validation.

Keywords: Copper; Fluorescence; IVIS spectrum; In vivo imaging; Ion; Metal; Near-infrared; Wilson’s disease.

MeSH terms

Animals
Biosensing Techniques
Copper / metabolism*
Fluorescent Dyes / administration & dosage*
Fluorescent Dyes / chemistry
Mice
Molecular Imaging
Whole Body Imaging / methods*

Substances

Fluorescent Dyes
Copper