Anti-fungal potential of ozone against some dermatophytes

Braz J Microbiol. 2016 Jul-Sep;47(3):697-702. doi: 10.1016/j.bjm.2016.04.014. Epub 2016 Apr 21.


Dermatophytes are classified in three genera, Epidermophyton, Microsporum and Trichophyton. They have the capacity to invade keratinized tissue to produce a cutaneous infection known as dermatophytoses. This investigation was performed to study the effect of gaseous ozone and ozonized oil on three specific properties of six different dermatophytes. These properties included sporulation, mycelia leakage of sugar and nutrients and the activity of their hydrolytic enzymes. Generally, ozonized oil was found to be more efficacious than gaseous ozone. Microsporum gypseum and Microsporum canis were the most susceptible, while Trichophyton interdigitale and T. mentagrophytes were relatively resistant. The study revealed a steady decline in spore production of M. gypseum and M. canis on application of ozonated oil. An increase in leakage of electrolytes and sugar was noticed after treatment with ozonized oil in the case of M. gypseum, M. canis, T. interdigitale, T. mentagrophytes and T. rubrum. The results also revealed loss in urease, amylase, alkaline phosphatase, lipase and keratinase enzyme producing capacity of the investigated fungi.

Keywords: Dermatophytes; Dermatophytosis; Hydrolytic enzymes; Ozone; Ozonized oil.

MeSH terms

  • Antifungal Agents / pharmacology*
  • Arthrodermataceae / drug effects*
  • Arthrodermataceae / physiology
  • Carbohydrate Metabolism / drug effects
  • Electrolytes / metabolism
  • Enzyme Activation
  • Fungal Proteins / metabolism
  • Humans
  • Mycelium
  • Ozone / pharmacology*
  • Permeability
  • Spores, Fungal / drug effects


  • Antifungal Agents
  • Electrolytes
  • Fungal Proteins
  • Ozone