A novel fluorescent probe for rapid and sensitive detection of hydrogen sulfide in living cells

Jian Pan; Junchao Xu; Youlai Zhang; Liang Wang; Caiqin Qin; Lintao Zeng; Yue Zhang

doi:10.1016/j.saa.2016.05.054

A novel fluorescent probe for rapid and sensitive detection of hydrogen sulfide in living cells

Spectrochim Acta A Mol Biomol Spectrosc. 2016 Nov 5:168:132-138. doi: 10.1016/j.saa.2016.05.054. Epub 2016 Jun 2.

Authors

Jian Pan¹, Junchao Xu¹, Youlai Zhang², Liang Wang², Caiqin Qin³, Lintao Zeng⁴, Yue Zhang⁵

Affiliations

¹ Department of Chemistry and Material Sciences, Hubei Engineering University, Hubei, Xiaogan 432000, PR China; School of Chemistry & Chemical Engineering, Tianjin University of Technology, Tianjin 300384, PR China.
² School of Chemistry & Chemical Engineering, Tianjin University of Technology, Tianjin 300384, PR China.
³ Department of Chemistry and Material Sciences, Hubei Engineering University, Hubei, Xiaogan 432000, PR China.
⁴ Department of Chemistry and Material Sciences, Hubei Engineering University, Hubei, Xiaogan 432000, PR China; School of Chemistry & Chemical Engineering, Tianjin University of Technology, Tianjin 300384, PR China. Electronic address: zlt1981@126.com.
⁵ School of Chemistry & Chemical Engineering, Tianjin University of Technology, Tianjin 300384, PR China. Electronic address: zhang5601@163.com.

PMID: 27289349
DOI: 10.1016/j.saa.2016.05.054

Abstract

A novel fluorescent probe for H2S was developed based on a far-red emitting indole-BODIPY, which was decorated with morpholine and 2,4-dinitrobenzenesulfonyl (DNBS) group. This probe showed rapid response (t1/2=3min), high selectivity and sensitivity for H2S with significant colorimetric and fluorescence OFF-ON signals, which was triggered by cleavage of 2,4-dinitrobenzenesulfonyl group. This probe could quantitatively detect the concentrations of H2S ranging from 0 to 60μM, and the detection of limit was found to be as low as 26nM. Cell imaging results indicated that the probe could detect and visualize H2S in the living cells.

Keywords: BODIPY; Fluorescent probe; Hydrogen sulfide imaging.

MeSH terms

Boron Compounds / chemistry*
Fluorescent Dyes / chemistry*
HeLa Cells
Humans
Hydrogen Sulfide / analysis*
Indoles / chemistry*
Microscopy, Fluorescence / methods
Optical Imaging / methods*
Spectrometry, Fluorescence / methods

Substances

4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
Boron Compounds
Fluorescent Dyes
Indoles
Hydrogen Sulfide