Ultraviolet shadowing nucleic acids on nylon membranes

Anal Biochem. 1989 Apr;178(1):41-2. doi: 10.1016/0003-2697(89)90353-9.


We describe a method for the direct visualization of nucleic acids on nylon membranes. Nylon is weakly fluorescent under short wave ultraviolet light allowing membrane-bound nucleic acids to be detected with a sensitivity of 10 ng. This procedure involves no staining or destaining of the gels prior to transfer, does not require duplicate sample lanes or blots, and does not interfere with transfer of the nucleic acid to the membrane or subsequent hybridization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis, Agar Gel
  • Nucleic Acids / analysis*
  • Nylons / radiation effects
  • Ultraviolet Rays*


  • Nucleic Acids
  • Nylons