Acute lung injury induced by whole gastric fluid: hepatic acute phase response contributes to increase lung antiprotease protection

Respir Res. 2016 Jun 14;17(1):71. doi: 10.1186/s12931-016-0379-7.


Background: Gastric contents aspiration in humans is a risk factor for severe respiratory failure with elevated mortality. Although aspiration-induced local lung inflammation has been studied in animal models, little is known about extrapulmonary effects of aspiration. We investigated whether a single orotracheal instillation of whole gastric fluid elicits a liver acute phase response and if this response contributes to enrich the alveolar spaces with proteins having antiprotease activity.

Methods: In anesthetized Sprague-Dawley rats receiving whole gastric fluid, we studied at different times after instillation (4 h -7 days): changes in blood cytokines and acute phase proteins (fibrinogen and the antiproteases alpha1-antitrypsin and alpha2-macroglobulin) as well as liver mRNA expression of the two antiproteases. The impact of the systemic changes on lung antiprotease defense was evaluated by measuring levels and bioactivity of antiproteases in broncho-alveolar lavage fluid (BALF). Markers of alveolar-capillary barrier derangement were also studied. Non-parametric ANOVA (Kruskall-Wallis) and linear regression analysis were used.

Results: Severe peribronchiolar injury involving edema, intra-alveolar proteinaceous debris, hemorrhage and PMNn cell infiltration was seen in the first 24 h and later resolved. Despite a large increase in several lung cytokines, only IL-6 was found elevated in blood, preceding increased liver expression and blood concentration of both antiproteases. These changes, with an acute phase response profile, were significantly larger for alpha2-macroglobulin (40-fold increment in expression with 12-fold elevation in blood protein concentration) than for alpha1-antitrypsin (2-3 fold increment in expression with 0.5-fold elevation in blood protein concentration). Both the increment in capillary-alveolar antiprotease concentration gradient due to increased antiprotease liver synthesis and a timely-associated derangement of the alveolar-capillary barrier induced by aspiration, contributed a 58-fold and a 190-fold increase in BALF alpha1-antitrypsin and alpha2-macroglobulin levels respectively (p < 0.001).

Conclusions: Gastric contents-induced acute lung injury elicits a liver acute phase response characterized by increased mRNA expression of antiproteases and elevation of blood antiprotease concentrations. Hepatic changes act in concert with derangement of the alveolar capillary barrier to enrich alveolar spaces with antiproteases. These findings may have significant implications decreasing protease burden, limiting injury in this and other models of acute lung injury and likely, in recurrent aspiration.

Keywords: Acute phase proteins; Alveolar-capillary barrier; Aspiration; Liver acute phase response; Lung antiprotease defense; α1-antitrypsin; α2-macroglobulin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Lung Injury / blood
  • Acute Lung Injury / enzymology*
  • Acute Lung Injury / etiology
  • Acute Lung Injury / pathology
  • Acute-Phase Reaction / blood
  • Acute-Phase Reaction / enzymology*
  • Acute-Phase Reaction / etiology
  • Acute-Phase Reaction / pathology
  • Animals
  • Blood-Air Barrier / enzymology
  • Blood-Air Barrier / pathology
  • Disease Models, Animal
  • Enzyme Induction
  • Inflammation Mediators / blood
  • Interleukin-6 / blood
  • Liver / metabolism*
  • Male
  • Pregnancy-Associated alpha 2-Macroglobulins / biosynthesis*
  • Pregnancy-Associated alpha 2-Macroglobulins / genetics
  • Pulmonary Alveoli / enzymology*
  • Pulmonary Alveoli / pathology
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Rats, Sprague-Dawley
  • Respiratory Aspiration of Gastric Contents / complications*
  • Time Factors
  • alpha 1-Antitrypsin / biosynthesis*
  • alpha 1-Antitrypsin / blood
  • alpha 1-Antitrypsin / genetics


  • Inflammation Mediators
  • Interleukin-6
  • Pregnancy-Associated alpha 2-Macroglobulins
  • RNA, Messenger
  • alpha 1-Antitrypsin