Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy

Chem Commun (Camb). 2016 Jun 28;52(54):8405-8. doi: 10.1039/c6cc03119f.


Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.

MeSH terms

  • Flavoproteins / metabolism*
  • Light*
  • Microscopy, Electron*
  • Photosensitizing Agents / metabolism*
  • Singlet Oxygen / metabolism


  • Flavoproteins
  • Photosensitizing Agents
  • Singlet Oxygen