Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Apr 23;9(4):e32656.
doi: 10.5812/jjm.32656. eCollection 2016 Apr.

The Ciprofloxacin Impact on Biofilm Formation by Proteus Mirabilis and P. Vulgaris Strains

Joanna Kwiecinska-Pirog  1 , Krzysztof Skowron  1 , Wojciech Bartczak  1 , Eugenia Gospodarek-Komkowska  1
Affiliations

The Ciprofloxacin Impact on Biofilm Formation by Proteus Mirabilis and P. Vulgaris Strains

Joanna Kwiecinska-Pirog et al. Jundishapur J Microbiol. .

Abstract

Background: Proteus spp. bacilli belong to opportunistic human pathogens, which are primarily responsible for urinary tract and wound infections. An important virulence factor is their ability to form biofilms that greatly reduce the effectiveness of antibiotics in the site of infection.

Objectives: The aim of this study was to determine the value of the minimum concentration of ciprofloxacin that eradicates a biofilm of Proteus spp. strains.

Materials and methods: A biofilm formation of 20 strains of P. mirabilis and 20 strains of P. vulgaris were evaluated by a spectrophotometric method using 0.1% 2, 3, 5-Triphenyl-tetrazolium chloride solution (TTC, AVANTORTM). On the basis of the results of the absorbance of the formazan, a degree of reduction of biofilm and minimum biofilm eradication (MBE) values of MBE50 and MBE90 were determined.

Results: All tested strains formed a biofilm. A value of 1.0 μg/mL ciprofloxacin is MBE50 for the strains of both tested species. An MBE90 value of ciprofloxacin for isolates of P. vulgaris was 2 μg/mL and for P. mirabilis was 512 μg/mL.

Conclusions: Minimum biofilm eradication values of ciprofloxacin obtained in the study are close to the values of the minimal inhibition concentration (MIC).

Keywords: Biofilm; Ciprofloxacin; Proteus spp.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.. The Origin of Proteus spp. Strains
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Manos J, Belas R. The Genera Proteus, Providencia, and Morganella. 2006:245–69. doi: 10.1007/0-387-30746-x_12. - DOI
    1. Rozalski A, Kwil I, Torzewska A, Baranowska M, Staczek P. Proteus bacilli: features and virulence factors. Postepy Hig Med Dosw (Online). 2007;61:204–19. - PubMed
    1. Jacobsen SM, Stickler DJ, Mobley HLT, Shirtliff ME. Complicated Catheter-Associated Urinary Tract Infections Due to Escherichia coli and Proteus mirabilis. Clinical Microbiology Reviews. 2008;21(1):26–59. doi: 10.1128/cmr.00019-07. - DOI - PMC - PubMed
    1. Billewicz-Kraczkowska A, Kulik-Rechberger B, Furmaga-Jabłońska, W. A noninvasive treatment of multiple brain abscesses in the course of neonatal cerebrospinal meningitis with Proteus mirabilis as etiological agent : A case study. Child Neurology. 2007;16(31):51–4.
    1. Reslinski, A., Mikucka, A., Szczęsny, W., Szmytkowski, J., Gospodarek, E., Dabrowiecki, S. Wykrywanie biofilmu in vivo na powierzchni siatki chirurgicznej -opis przypadku. Chir Pol. 2008;10(1-2):181–8.

LinkOut - more resources