Purification of a lipoprotein lipase-inhibiting protein produced by a melanoma cell line associated with cancer cachexia

Biochem Biophys Res Commun. 1989 May 15;160(3):1085-92. doi: 10.1016/s0006-291x(89)80114-7.


A human melanoma cell line, SEKI, induces severe cachexia in tumor-bearing nude mice. A factor with the ability to inhibit lipoprotein lipase (LPL) was isolated from the conditioned medium of this cell line. This factor was 40-K-dalton protein, and designated temporarily as melanoma-derived LPL inhibitor (MLPLI). Amino acid sequencing revealed that the amino-terminal portion consists of SPLPITPV-AT--IR-P. Unexpectedly, the sequence, as far as determined, was identical to those of leukemia inhibitory factor (LIF), suggesting that MLPLI is a protein closely related to LIF. The findings that MLPLI inhibits LPL activity and that MLPLI is produced by human cancer cells inducing cancer cachexia also suggest that this protein is a candidate for the factor responsible for cancer cachexia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cachexia / etiology
  • Cachexia / metabolism*
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Humans
  • Lipoprotein Lipase / antagonists & inhibitors*
  • Melanoma / complications
  • Melanoma / metabolism*
  • Mice
  • Mice, Nude
  • Molecular Sequence Data
  • Neoplasm Transplantation
  • Protein Biosynthesis
  • Proteins / isolation & purification*
  • Tumor Cells, Cultured


  • Proteins
  • Lipoprotein Lipase