Cytogenetic analysis of in vitro karyotype evolution in a cell line established from nonmalignant human mammary epithelium

Cancer Genet Cytogenet. 1989 May;39(1):103-18. doi: 10.1016/0165-4608(89)90236-7.


Cytogenetic analysis was carried out on six passages (pass. 16, 19, 21, 25, 34, and 47) of the nontumorigenic epithelial cell line, HMT-3522, established from a fibrocystic lesion of the human breast. Minor chromosome abnormalities were present in the first passage (pass. 16) available for study, and limited cytogenetic progression was observed during the in vitro growth. A modal chromosome number of 45 chromosomes was found in all passages. Each passage contained 4-5 marker chromosomes. Three markers were consistently present in all passages studied. During in vitro growth two markers were gained and two markers were lost from the stemline karyotype. The two latest passages studied had identical karyotypes: 45,XX, del(1)(q44----p32:),t(5;14)(14p13----14q32::5q22----++ +5q35),t(6;8;12;17)(8p23---- 8q24::6p21.1----6p23;12q24----12p13::6p23- --- 6p25;17p13----17q25::6q11----6q27). The present study demonstrates chromosome abnormalities and karyotypic evolution in a nontumorigenic (in nude mice) and noninvasive (in vitro tested) cell line established from nonmalignant epithelial breast tissue. The results are discussed in relation to gene amplification, double minutes and oncogene localization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast / pathology
  • Breast / ultrastructure*
  • Cell Line
  • Chromosome Aberrations*
  • Epithelium / pathology
  • Epithelium / ultrastructure
  • Fibrocystic Breast Disease / genetics*
  • Fibrocystic Breast Disease / pathology
  • Genetic Markers
  • Humans
  • Karyotyping
  • Middle Aged


  • Genetic Markers