Physicochemical, agronomical and microbiological evaluation of alternative growing media for the production of rapini (Brassica rapa L.) microgreens

J Sci Food Agric. 2017 Mar;97(4):1212-1219. doi: 10.1002/jsfa.7852. Epub 2016 Jul 11.

Abstract

Background: Peat-based mixes and synthetic mats are the main substrates used for microgreens production. However, both are expensive and non-renewable. Recycled fibrous materials may represent low-cost and renewable alternative substrates. Recycled textile-fiber (TF; polyester, cotton and polyurethane traces) and jute-kenaf-fiber (JKF; 85% jute, 15% kenaf-fibers) mats were characterized and compared with peat and Sure to Grow® (Sure to Grow, Beachwood, OH, USA; http://suretogrow.com) (STG; 100% polyethylene-terephthalate) for the production of rapini (Brassica rapa L.; Broccoletto group) microgreens.

Results: All substrates had suitable physicochemical properties for the production of microgreens. On average, microgreens fresh yield was 1502 g m-2 in peat, TF and JKF, and was 13.1% lower with STG. Peat-grown microgreen shoots had a higher concentration of K+ and SO42- and a two-fold higher NO3- concentration [1959 versus 940 mg kg-1 fresh weight (FW)] than those grown on STG, TF and JKF. At harvest, substrates did not influence microgreens aerobic bacterial populations (log 6.48 CFU g-1 FW). Peat- and JKF-grown microgreens had higher yeast-mould counts than TF- and STG microgreens (log 2.64 versus 1.80 CFU g-1 FW). Peat-grown microgreens had the highest population of Enterobacteriaceae (log 5.46 ± 0.82 CFU g-1 ) and Escherichia coli (log 1.46 ± 0.15 CFU g-1 ). Escherichia coli was not detected in microgreens grown on other media.

Conclusion: TF and JKF may be valid alternatives to peat and STG because both ensured a competitive yield, low nitrate content and a similar or higher microbiological quality. © 2016 Society of Chemical Industry.

Keywords: Brassica rapa L; Enterobacteriaceae; Escherichia coli; broccoli raab microgreens; peat alternative; recycled fibrous materials.

MeSH terms

  • Agriculture / methods*
  • Biomass
  • Brassica rapa / growth & development*
  • Brassica rapa / metabolism
  • Colony Count, Microbial
  • Corchorus
  • Cotton Fiber
  • Culture Media / chemistry*
  • Enterobacteriaceae / growth & development
  • Escherichia coli / growth & development
  • Food Microbiology*
  • Fungi / growth & development
  • Hibiscus
  • Humans
  • Nitrates / analysis
  • Phthalic Acids
  • Polyesters
  • Polyurethanes
  • Recycling
  • Seedlings / growth & development*
  • Seedlings / metabolism
  • Soil*
  • Textiles*
  • Yeasts / growth & development

Substances

  • Culture Media
  • Nitrates
  • Phthalic Acids
  • Polyesters
  • Polyurethanes
  • Soil
  • terephthalic acid