Abstract
Sialic acid-binding immunoglobulin-like lectin-7 (Siglec-7) is an inhibitory receptor expressed on natural killer (NK) cells. In this study, we investigated the relationship between Siglec-7 expression and NK cell functions. Siglec-7 was highly expressed on NK cells and was preferentially expressed by mature NK cells from peripheral blood of healthy adults. Siglec-7(+) NK cells displayed higher levels of activating receptors CD38, CD16, DNAM1, NKp30 and NKp46, but lower levels of inhibitory receptors such as NKG2A and CD158b, compared with Siglec-7(-) NK cells. Functional tests showed that Siglec-7(+) NK cells displayed more CD107a degranulation and IFN-γ production than Siglec-7(-) NK cells. Siglec-7 inhibited NK cell functions when interacting with specific antibodies. These data suggest that Siglec-7 defines a highly functional NK cell subset and suppresses NK cell-mediated functions when cross-linked with specific antibodies.
© 2016 The Foundation for the Scandinavian Journal of Immunology.
MeSH terms
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ADP-ribosyl Cyclase 1 / genetics
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ADP-ribosyl Cyclase 1 / immunology
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Antigens, Differentiation, Myelomonocytic / genetics
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Antigens, Differentiation, Myelomonocytic / immunology*
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Antigens, Differentiation, T-Lymphocyte / genetics
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Antigens, Differentiation, T-Lymphocyte / immunology
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Cell Degranulation
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Cell Lineage
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GPI-Linked Proteins / genetics
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GPI-Linked Proteins / immunology
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Gene Expression Regulation
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Humans
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Immunity, Cellular*
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Immunophenotyping
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Interferon-gamma / genetics
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Interferon-gamma / immunology
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Killer Cells, Natural / cytology
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Killer Cells, Natural / immunology*
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Lectins / genetics
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Lectins / immunology*
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Lysosomal-Associated Membrane Protein 1 / genetics
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Lysosomal-Associated Membrane Protein 1 / immunology
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / immunology
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NK Cell Lectin-Like Receptor Subfamily C / genetics
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NK Cell Lectin-Like Receptor Subfamily C / immunology
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Natural Cytotoxicity Triggering Receptor 1 / genetics
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Natural Cytotoxicity Triggering Receptor 1 / immunology
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Natural Cytotoxicity Triggering Receptor 3 / genetics
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Natural Cytotoxicity Triggering Receptor 3 / immunology
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Receptors, IgG / genetics
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Receptors, IgG / immunology
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Receptors, KIR2DL3 / genetics
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Receptors, KIR2DL3 / immunology
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Signal Transduction
Substances
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Antigens, Differentiation, Myelomonocytic
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Antigens, Differentiation, T-Lymphocyte
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CD226 antigen
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FCGR3B protein, human
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GPI-Linked Proteins
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IFNG protein, human
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KLRC1 protein, human
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Lectins
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Lysosomal-Associated Membrane Protein 1
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Membrane Glycoproteins
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NCR1 protein, human
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NCR3 protein, human
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NK Cell Lectin-Like Receptor Subfamily C
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Natural Cytotoxicity Triggering Receptor 1
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Natural Cytotoxicity Triggering Receptor 3
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Receptors, IgG
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Receptors, KIR2DL3
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SIGLEC7 protein, human
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Interferon-gamma
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CD38 protein, human
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ADP-ribosyl Cyclase 1