Mycobacterium tuberculosis High-Throughput Screening

Methods Mol Biol. 2016;1439:181-95. doi: 10.1007/978-1-4939-3673-1_12.

Abstract

High-throughput screening is a valuable way to identify hit compounds that combined with a robust medicinal chemistry program could lead to the identification of new antibiotics. Here, we discuss our method for screening large compound libraries with virulent Mycobacterium tuberculosis, possibly one of the more difficult bacteria to use because of its slow growth and assignment to Biosafety Level-3 by the CDC and NIH. The principles illuminated here, however, are relevant to the execution of most bacteria high-throughput screens.

Keywords: Antibacterial; Antibiotics; Antimicrobial; Antitubercular; H37Rv; HTS; High-throughput screening; Mtb; Mycobacterium tuberculosis; TB; Tuberculosis.

MeSH terms

  • Animals
  • Antitubercular Agents / pharmacology*
  • Cell Culture Techniques / methods
  • Chlorocebus aethiops
  • High-Throughput Screening Assays / methods*
  • Humans
  • Microbial Sensitivity Tests / methods*
  • Mycobacterium tuberculosis / drug effects*
  • Mycobacterium tuberculosis / growth & development
  • Small Molecule Libraries / pharmacology
  • Tuberculosis / drug therapy*
  • Tuberculosis / microbiology
  • Vero Cells

Substances

  • Antitubercular Agents
  • Small Molecule Libraries