Effect of Porphyrin Sensitizer MgTPPS4 on Cytoskeletal System of HeLa Cell Line-Microscopic Study

Cell Biochem Biophys. 2016 Sep;74(3):419-25. doi: 10.1007/s12013-016-0746-5. Epub 2016 Jun 21.


Metalloporphyrins are an important group of sensitizers with a porphyrin skeleton. Their photophysical properties are significantly affected by the nature of the central ion. In this work, we focus on the mechanical properties of a cervix carcinoma cell line which underwent photodynamic treatment (PDT) with MgTPPS4 photosensitzer. Atomic force microscopy alongside confocal microscopy was used to quantify and qualify the structural characteristics before and after PDT. Cells before PDT showed a fine actin network and higher elasticity with the median of Young modulus 12.2 kPa. After PDT, the median of Young modulus was 13.4 kPa and a large redistribution in the actin network was observed.

Keywords: Atomic force microscopy; Cytoskeleton; F-actin; Mechanical properties; Photodynamic treatment.

MeSH terms

  • Cytoskeleton / drug effects*
  • Cytoskeleton / physiology
  • Cytoskeleton / radiation effects
  • Elastic Modulus
  • HeLa Cells
  • Humans
  • Light
  • Metalloporphyrins / chemistry
  • Metalloporphyrins / pharmacology*
  • Microscopy, Atomic Force
  • Microscopy, Confocal
  • Photosensitizing Agents / chemistry
  • Photosensitizing Agents / pharmacology*


  • Metalloporphyrins
  • Photosensitizing Agents
  • magnesium 5,10,15,20-tetrakis(4-sulphophenyl)-porphine dodecahydrate