The large viral RNA segment of California serogroup bunyaviruses encodes the large viral protein

J Gen Virol. 1989 Jan;70 ( Pt 1):223-8. doi: 10.1099/0022-1317-70-1-223.

Abstract

Reassortant bunyaviruses derived from two members of the California serogroup (La Crosse/original and Tahyna/181-57) viruses were used to demonstrate that the large Mr viral protein (L) is encoded by the L RNA segment. Radiolabelled viral proteins were analysed by discontinuous SDS-PAGE. The L protein of La Crosse virus was observed to migrate ahead of its Tahyna virus counterpart when electrophoresed through a 5% acrylamide resolving gel. Among the reassortant viruses, the L protein phenotype segregated with the viral L RNA segment. After confirming the genotype of the viruses used in this study, it was concluded that the L RNA species of California serogroup viruses codes for the L protein, the presumed viral polymerase.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bunyaviridae / genetics*
  • Cell Line
  • DNA Probes
  • Electrophoresis, Polyacrylamide Gel
  • Encephalitis Virus, California / classification
  • Encephalitis Virus, California / genetics*
  • Genotype
  • Nucleic Acid Hybridization
  • RNA, Viral / genetics*
  • Viral Proteins / analysis
  • Viral Proteins / genetics*

Substances

  • DNA Probes
  • RNA, Viral
  • Viral Proteins