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. 2016;33(4):435-452.
doi: 10.14573/altex.1601281. Epub 2016 Jun 21.

Advancing Toxicology Research Using in Vivo High Throughput Toxicology With Small Fish Models

Free PMC article

Advancing Toxicology Research Using in Vivo High Throughput Toxicology With Small Fish Models

Antonio Planchart et al. ALTEX. .
Free PMC article


Small freshwater fish models, especially zebrafish, offer advantages over traditional rodent models, including low maintenance and husbandry costs, high fecundity, genetic diversity, physiology similar to that of traditional biomedical models, and reduced animal welfare concerns. The Collaborative Workshop on Aquatic Models and 21st Century Toxicology was held at North Carolina State University on May 5-6, 2014, in Raleigh, North Carolina, USA. Participants discussed the ways in which small fish are being used as models to screen toxicants and understand mechanisms of toxicity. Workshop participants agreed that the lack of standardized protocols is an impediment to broader acceptance of these models, whereas development of standardized protocols, validation, and subsequent regulatory acceptance would facilitate greater usage. Given the advantages and increasing application of small fish models, there was widespread interest in follow-up workshops to review and discuss developments in their use. In this article, we summarize the recommendations formulated by workshop participants to enhance the utility of small fish species in toxicology studies, as well as many of the advances in the field of toxicology that resulted from using small fish species, including advances in developmental toxicology, cardiovascular toxicology, neurotoxicology, and immunotoxicology. We alsoreview many emerging issues that will benefit from using small fish species, especially zebrafish, and new technologies that will enable using these organisms to yield results unprecedented in their information content to better understand how toxicants affect development and health.

Keywords: 21st century toxicology; alternatives; aquatic models.

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    1. Agranoff BW, Davis RE, Brink JJ. Memory fixation in the goldfish. Proc Natl Acad Sci U S A. 1965;54:788–793. - DOI - PMC - PubMed
    1. Asnani A, Peterson RT. The zebrafish as a tool to identify novel therapies for human cardiovascular disease. Dis Model Mech. 2014;7:763–767. - DOI - PMC - PubMed
    1. Attene-Ramos MS, Huang R, Sakamuru S, et al. Systematic study of mitochondrial toxicity of environmental chemicals using quantitative high throughput screening. Chem Res Toxicol. 2013a;26:1323–1332. - DOI - PMC - PubMed
    1. Attene-Ramos MS, Miller N, Huang R, et al. The Tox21 robotic platform for the assessment of environmental chemicals – from vision to reality. Drug Discov Today. 2013b;18:716–723. - DOI - PMC - PubMed
    1. Auer TO, Duroure K, De Cian A, et al. Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair. Genome Res. 2014;24:142–153. - DOI - PMC - PubMed