Comparison of the Modified-Hodge test, Carba NP test, and carbapenem inactivation method as screening methods for carbapenemase-producing Enterobacteriaceae

Kageto Yamada; Machiko Kashiwa; Katsumi Arai; Noriyuki Nagano; Ryoichi Saito

doi:10.1016/j.mimet.2016.06.019

Comparison of the Modified-Hodge test, Carba NP test, and carbapenem inactivation method as screening methods for carbapenemase-producing Enterobacteriaceae

J Microbiol Methods. 2016 Sep:128:48-51. doi: 10.1016/j.mimet.2016.06.019. Epub 2016 Jun 18.

Authors

Kageto Yamada¹, Machiko Kashiwa², Katsumi Arai², Noriyuki Nagano³, Ryoichi Saito⁴

Affiliations

¹ Department of Clinical Laboratory, Tokyo Metropolitan Health and Medical Treatment Corporation Toshima Hospital, Tokyo, Japan. Electronic address: kageto_yamada@tokyo-hmt.jp.
² Department of Clinical Laboratory, Tokyo Metropolitan Health and Medical Treatment Corporation Toshima Hospital, Tokyo, Japan.
³ Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine, Nagano, Japan; Division of Clinical Microbiology, Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University School of Medicine, Nagano, Japan.
⁴ Department of Microbiology and Immunology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan.

PMID: 27329263
DOI: 10.1016/j.mimet.2016.06.019

Abstract

We compared three screening methods for carbapenemase-producing Enterobacteriaceae. While the Modified-Hodge test and Carba NP test produced false-negative results for OXA-48-like and mucoid NDM producers, the carbapenem inactivation method (CIM) showed positive results for these isolates. Although the CIM required cultivation time, it is well suited for general clinical laboratories.

Keywords: Carba NP test; Carbapenem inactivation method; Carbapenemase-producing Enterobacteriaceae; Modified-Hodge test.

Publication types

Comparative Study

MeSH terms

Anti-Bacterial Agents
Bacterial Proteins / chemistry*
Carbapenems / chemistry
Enterobacteriaceae / classification
Enterobacteriaceae / enzymology
Enterobacteriaceae / isolation & purification*
Ertapenem
Imipenem / chemistry
Meropenem
Microbial Sensitivity Tests
Sensitivity and Specificity
Thienamycins / chemistry
beta-Lactamases / chemistry*
beta-Lactams / chemistry

Substances

Anti-Bacterial Agents
Bacterial Proteins
Carbapenems
Thienamycins
beta-Lactams
Imipenem
beta-Lactamases
carbapenemase
Meropenem
Ertapenem