Differential hypomethylation of the c-myc protooncogene in bladder cancers at different stages and grades

J Urol. 1989 Jul;142(1):146-9. doi: 10.1016/s0022-5347(17)38700-1.


The 5-methylcytosine content of c-myc proto-oncogene DNA obtained from samples of normal bladders and of bladders with transitional cell carcinoma of different stage and grade was analyzed. Five CCGG sites (from the third coding region to the 3' flanking region of the gene) which showed different methylation in normal and neoplastic samples were identified. The overall c-myc methylation levels were significantly reduced in carcinomas. Furthermore, a significant correlation between disease invasiveness and methylation level was found. Nevertheless, in each group of patients with the same histological grade, heterogeneity has been observed. This might be suggestive of different prognosis of the disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5-Methylcytosine
  • Autoradiography
  • Blotting, Southern
  • Carcinoma, Transitional Cell / genetics*
  • Carcinoma, Transitional Cell / pathology
  • Cytosine / analogs & derivatives
  • Cytosine / metabolism
  • DNA, Neoplasm / metabolism*
  • Humans
  • Methylation
  • Neoplasm Invasiveness
  • Neoplasm Staging
  • Oncogenes*
  • Prognosis
  • Proto-Oncogene Mas
  • Proto-Oncogenes*
  • Urinary Bladder Neoplasms / genetics*
  • Urinary Bladder Neoplasms / pathology


  • DNA, Neoplasm
  • MAS1 protein, human
  • Proto-Oncogene Mas
  • 5-Methylcytosine
  • Cytosine