Unraveling the rat blood genome-wide transcriptome after oral administration of lavender oil by a two-color dye-swap DNA microarray approach

Motohide Hori; Hiroko Kubo; Junko Shibato; Tomomi Saito; Tetsuo Ogawa; Minoru Wakamori; Yoshinori Masuo; Seiji Shioda; Randeep Rakwal

doi:10.1016/j.gdata.2016.05.005

Unraveling the rat blood genome-wide transcriptome after oral administration of lavender oil by a two-color dye-swap DNA microarray approach

Genom Data. 2016 May 12:8:139-45. doi: 10.1016/j.gdata.2016.05.005. eCollection 2016 Jun.

Authors

Motohide Hori¹, Hiroko Kubo², Junko Shibato³, Tomomi Saito⁴, Tetsuo Ogawa⁵, Minoru Wakamori¹, Yoshinori Masuo⁶, Seiji Shioda³, Randeep Rakwal⁷

Affiliations

¹ Department of Oral Biology, Graduate School of Dentistry, Tohoku University, Sendai 980-8575, Japan.
² Department of Anatomy I, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan; Oriental Aromatherapy College, 5-22-9 Kameari, Katsushika, Tokyo 125-0061, Japan.
³ Department of Anatomy I, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan; Global Research Center for Innovative Life Science, Peptide Drug Innovation, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 4-41 Ebara 2-chome, Shinagawa, Tokyo 142-8501, Japan.
⁴ Department of Anatomy I, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan.
⁵ Department of Anatomy I, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan; Department of Physiology, Saitama Medical University, 38 Morohongo Moroyama-machi, Iruma-gun, Saitama 350-0495, Japan.
⁶ Laboratory of Neuroscience, Department of Biology, Faculty of Science, Toho University, Funabashi, Chiba 274-8510, Japan.
⁷ Department of Anatomy I, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan; Global Research Center for Innovative Life Science, Peptide Drug Innovation, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 4-41 Ebara 2-chome, Shinagawa, Tokyo 142-8501, Japan; Faculty of Health and Sport Sciences and Tsukuba International Academy for Sport Studies (TIAS), University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8574, Ibaraki, Japan.

Abstract

Lavender oil (LO) is a commonly used essential oil in aromatherapy as non-traditional medicine. With an aim to demonstrate LO effects on the body, we have recently established an animal model investigating the influence of orally administered LO in rat tissues, genome-wide. In this brief, we investigate the effect of LO ingestion in the blood of rat. Rats were administered LO at usual therapeutic dose (5 mg/kg) in humans, and following collection of the venous blood from the heart and extraction of total RNA, the differentially expressed genes were screened using a 4 × 44-K whole-genome rat chip (Agilent microarray platform; Agilent Technologies, Palo Alto, CA, USA) in conjunction with a two-color dye-swap approach. A total of 834 differentially expressed genes in the blood were identified: 362 up-regulated and 472 down-regulated. These genes were functionally categorized using bioinformatics tools. The gene expression inventory of rat blood transcriptome under LO, a first report, has been deposited into the Gene Expression Omnibus (GEO): GSE67499. The data will be a valuable resource in examining the effects of natural products, and which could also serve as a human model for further functional analysis and investigation.

Keywords: Gene expression; Lavender oil ingestion; Rat; Total RNA extraction; Whole blood.