Bilirubin isomer distribution in jaundiced neonates during phototherapy with LED light centered at 497 nm (turquoise) vs. 459 nm (blue)

Pediatr Res. 2016 Oct;80(4):511-5. doi: 10.1038/pr.2016.115. Epub 2016 May 25.


Background: Phototherapy using blue light is the treatment of choice worldwide for neonatal hyperbilirubinemia. However, treatment with turquoise light may be a desirable alternative. Therefore, the aim of this randomized, controlled study was to compare the bilirubin isomer distribution in serum of jaundiced neonates after 24 h of therapy with narrow-band (LED) light centered at 497 nm (turquoise) vs. 459 nm (blue), of essentially equal irradiance.

Materials: Eighty-three neonates (≥33 wk gestational age) with uncomplicated hyperbilirubinemia were included in the study. Forty neonates were exposed to light centered at 497 nm and 43 infants with light centered at 459 nm. Irradiances were 5.2 × 10(15) and 5.1 × 10(15) photons/cm(2)/s, respectively.

Results: After 24 h of treatment no significant differences in serum concentrations of total bilirubin isomers and Z,Z-bilirubin were observed between the 2 groups. Interestingly, concentrations of Z,E-bilirubin, and thus also total bilirubin isomers formed during therapy, were highest for infants receiving light centered at 459 nm, while the concentration of E,Z-bilirubin was highest for those receiving light centered at 497 nm. No significant difference was found between concentrations of E,Z-lumirubin.

Conclusion: Therapy with LED light centered at 497 nm vs. 459 nm, applied with equal irradiance on the infants, resulted in a different distribution of bilirubin isomers in serum.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Bilirubin / analogs & derivatives*
  • Bilirubin / blood*
  • Female
  • Gestational Age
  • Humans
  • Hyperbilirubinemia, Neonatal / therapy*
  • Infant, Newborn
  • Infant, Premature
  • Jaundice, Neonatal / blood*
  • Jaundice, Neonatal / therapy
  • Light
  • Male
  • Oxygen / chemistry
  • Phototherapy / methods*
  • Protein Isoforms
  • Time Factors


  • Protein Isoforms
  • lumirubin
  • Bilirubin
  • Oxygen