Antistress Effects of Rosa rugosa Thunb. on Total Sleep Deprivation-Induced Anxiety-Like Behavior and Cognitive Dysfunction in Rat: Possible Mechanism of Action of 5-HT6 Receptor Antagonist

J Med Food. 2016 Sep;19(9):870-81. doi: 10.1089/jmf.2016.3660. Epub 2016 Jun 22.


Our previous results suggest that the Rosa rugosa Thunb. (family Rosaceae) alleviates endurance exercise-induced stress by decreasing oxidative stress levels. This study aimed to screen and identify the physiological antistress effects of an extract of R. rugosa (RO) on sleep deprivation-induced anxiety-like behavior and cognitive tests (in vivo) and tested for hippocampal CORT and monoamine levels (ex vivo), corticosterone (CORT)-induced injury, N-methyl-d-aspartate (NMDA) receptor, and serotonin 6 (5-hydroxytryptamine 6, 5-HT6) receptor activities (in vitro) in search of active principles and underlying mechanisms of action. We confirmed the antistress effects of RO in a sleep-deprived stress model in rat and explored the underlying mechanisms of its action. In conclusion, an R. rugosa extract showed efficacy and potential for use as an antistress therapy to treat sleep deprivation through its antagonism of the 5-HT6 receptor and resulting inhibition of cAMP activity.

Keywords: 5-HT6 receptor; Rosa rugosa; cAMP inhibition; cognition; sleep deprivation; stress.

MeSH terms

  • Animals
  • Antioxidants / pharmacology
  • Antioxidants / therapeutic use
  • Anxiety / drug therapy
  • Anxiety / metabolism*
  • Behavior, Animal
  • Brain / drug effects
  • Brain / metabolism
  • Cognitive Dysfunction / drug therapy
  • Cognitive Dysfunction / metabolism*
  • Corticosterone / metabolism
  • Cyclic AMP / antagonists & inhibitors
  • Dopamine / metabolism
  • Male
  • Phytotherapy
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Rats, Sprague-Dawley
  • Receptors, N-Methyl-D-Aspartate / metabolism
  • Receptors, Serotonin / metabolism*
  • Rosa*
  • Serotonin / blood
  • Sleep / physiology
  • Sleep Deprivation / complications
  • Sleep Deprivation / metabolism
  • Sleep Deprivation / psychology*
  • Stress, Physiological / drug effects*


  • Antioxidants
  • Plant Extracts
  • Receptors, N-Methyl-D-Aspartate
  • Receptors, Serotonin
  • serotonin 6 receptor
  • Serotonin
  • Cyclic AMP
  • Dopamine
  • Corticosterone