Evaluation of different conditions and culture media for the recovery of Aeromonas spp. from water and shellfish samples

J Appl Microbiol. 2016 Sep;121(3):883-91. doi: 10.1111/jam.13210. Epub 2016 Jul 28.

Abstract

Aims: To perform a comparative study for determining the optimum culture method (direct plating or enrichment) and medium (ampicillin dextrin agar (ADA), starch ampicillin agar (SAA), bile salts irgasan brilliant green modified (BIBG-m)) for recovering Aeromonas species from water and shellfish samples.

Methods and results: By direct culture, Aeromonas was detected in 65% (13/20) of the water samples and in 54·5% (6/11) of the shellfish samples. However, when a pre-enrichment step was included, the number of positive water samples increased to 75% (15/20) and the ones of shellfish to 90·1% (10/11). The enriched culture significantly favoured (P < 0·05) the isolation of Aeromonas allosaccharophila from water, Aeromonas salmonicida from shellfish and Aeromonas caviae from both types of samples. The most specific (P < 0·05) culture medium for detecting Aeromonas from water was ADA. However, no differences were observed in the case of shellfish samples (P > 0·05). Isolation of Aeromonas media from water was favoured (P < 0·05) in the ADA medium, while SAA enhanced (P < 0·05) the isolation of Aer. salmonicida from shellfish.

Conclusions: The culture method and medium used influenced the recovery of some Aeromonas species from water and shellfish samples.

Significance and impact of the study: This fact should be considered in future prevalence studies to avoid overestimating the above mentioned Aeromonas species.

Keywords: Aeromonas; culture media; direct plating; enrichment; shellfish; water.

Publication types

  • Evaluation Study

MeSH terms

  • Aeromonas / growth & development*
  • Aeromonas / isolation & purification
  • Aeromonas / metabolism
  • Bacteriological Techniques / instrumentation
  • Bacteriological Techniques / methods*
  • Culture Media / chemistry
  • Culture Media / metabolism*
  • Fresh Water / microbiology*
  • Shellfish / microbiology*

Substances

  • Culture Media