Intramyocardial Adipose-Derived Stem Cell Transplantation Increases Pericardial Fat with Recovery of Myocardial Function after Acute Myocardial Infarction

PLoS One. 2016 Jun 23;11(6):e0158067. doi: 10.1371/journal.pone.0158067. eCollection 2016.


Intramyocardial injection of adipose-derived stem cells (ASC) with other cell types in acute myocardial infarction (AMI) animal models has consistently shown promising clinical regenerative capacities. We investigated the effects of intramyocardial injections of mouse ASC (mASC) with mouse endothelial cells (mEC) on left ventricular function and generation of pericardial fat in AMI rats. AMI rat models were created by ligating left anterior descending coronary artery and were randomly assigned into four groups: control (n = 10), mASC (n = 10), mEC (n = 10) and mASC+mEC (n = 10) via direct intramyocardial injections, and each rat received 1x106 cells around three peri-infarct areas. Echocardiography and cardiac positron emission tomography (PET) were compared at baseline and on 28 days after AMI. Changes in left ventricular ejection fraction measured by PET, increased significantly in mASC and mASC+mEC groups compared to mEC and control groups. Furthermore, significant decreases in fibrosis were confirmed after sacrifice on 28 days in mASC and mASC+mEC groups. Successful cell engraftment was confirmed by positive Y-Chromosome staining in the transplantation region. Pericardial fat increased significantly in mASC and mASC+mEC groups compared to control group, and pericardial fat was shown to originate from the AMI rat. mASC group expressed higher adiponectin and lower leptin levels in plasma than control group. In addition, pericardial fat from AMI rats demonstrated increased phospho-AMPK levels and reduced phospho-ACC levels. Intramyocardial mASC transplantation after AMI in rats increased pericardial fat, which might play a protective role in the recovery of myocardial function after ischemic myocardial damage.

MeSH terms

  • Actins / metabolism
  • Adipokines / metabolism
  • Adipose Tissue / cytology*
  • Animals
  • Biomarkers
  • Cardiac Rehabilitation*
  • Disease Models, Animal
  • Endothelial Cells / metabolism
  • Female
  • Fibrosis
  • Heart Function Tests
  • Immunophenotyping
  • Male
  • Mice
  • Myocardial Infarction / diagnosis
  • Myocardial Infarction / etiology
  • Myocardial Infarction / physiopathology*
  • Myocardial Infarction / therapy
  • Myocardium*
  • Neovascularization, Pathologic
  • Phenotype
  • Rats
  • Stem Cell Transplantation*
  • Stem Cells / cytology*
  • Stem Cells / metabolism


  • Actins
  • Adipokines
  • Biomarkers
  • alpha-smooth muscle actin, mouse

Grant support

This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare (Grant number: HI14C0209 and A120275), and by a grant of the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (No. NRF-2014R1A2A1A11051998), Republic of Korea.