Microarray Expression Profile of Circular RNAs in Heart Tissue of Mice with Myocardial Infarction-Induced Heart Failure

Cell Physiol Biochem. 2016;39(1):205-16. doi: 10.1159/000445617. Epub 2016 Jun 24.


Background/aims: Myocardial infarction (MI) is a serious complication of atherosclerosis associated with increasing mortality attributable to heart failure. This study is aimed to assess the global changes in and characteristics of the transcriptome of circular RNAs (circRNAs) in heart tissue during MI induced heart failure (HF).

Methods: Using a post-myocardial infarction (MI) model of HF in mice, we applied microarray assay to examine the transcriptome of circRNAs deregulated in the heart during HF. We confirmed the changes in circRNAs by quantitative PCR.

Results: We revealed and confirmed a number of circRNAs that were deregulated during HF, which suggests a potential role of circRNAs in HF.

Conclusions: The distinct expression patterns of circulatory circRNAs during HF indicate that circRNAs may actively respond to stress and thus serve as biomarkers of HF diagnosis and treatment.

MeSH terms

  • Animals
  • Cluster Analysis
  • Gene Expression Profiling / methods*
  • Heart Failure / etiology
  • Heart Failure / genetics*
  • Male
  • Mice, Inbred C57BL
  • MicroRNAs / genetics
  • Myocardial Infarction / complications*
  • Myocardium / metabolism*
  • Myocardium / pathology
  • Oligonucleotide Array Sequence Analysis / methods
  • RNA / classification
  • RNA / genetics*
  • RNA, Circular
  • Reverse Transcriptase Polymerase Chain Reaction


  • MicroRNAs
  • RNA, Circular
  • RNA