Differential pulse anodic stripping voltammetry for detection of As (III) by Chitosan-Fe(OH)3 modified glassy carbon electrode: A new approach towards speciation of arsenic

Suparna Saha; Priyabrata Sarkar

doi:10.1016/j.talanta.2016.05.053

Differential pulse anodic stripping voltammetry for detection of As (III) by Chitosan-Fe(OH)3 modified glassy carbon electrode: A new approach towards speciation of arsenic

Talanta. 2016 Sep 1:158:235-245. doi: 10.1016/j.talanta.2016.05.053. Epub 2016 May 24.

Authors

Suparna Saha¹, Priyabrata Sarkar²

Affiliations

¹ Biosensor Laboratory, Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 700 009, India.
² Biosensor Laboratory, Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 700 009, India. Electronic address: sarkarpriya@gmail.com.

PMID: 27343601
DOI: 10.1016/j.talanta.2016.05.053

Abstract

An efficient electrochemical sensor for As(III) was developed based on adsorption of arsenic on a specially modified electrodes at some applied potential and subsequent i) stripping at a fixed potential by anodic stripping voltammetry ii) analysis by generating surface plasmon resonance (SPR). The working glassy carbon electrode was modified by Chitosan-Fe(OH)3 composite and a reducing agent L-cysteine. The composite enhanced adsorption of As(III) and subsequent reduction to As(O) moieties and measurement by anodic stripping. The surface property of modified electrode was characterized by SEM, AFM, FTIR, XPS and electrochemistry was analyzed by impedance spectroscopy (EIS). Surface Plasmon resonance (SPR) was also employed to investigate the As(III) binding capability of polymer matrix. Several optimum voltammetric parameters e.g supporting electrolyte; 0.1M acetate buffer (pH 5.2) deposition potential, -0.9V; deposition time, 100s were established for anodic stripping voltammetry (ASV). A linear correlation was obtained in the range of 2-100ppb for ASV (R(2) 0.974) with limit of detection 0.072ppb. A variety of common coexistent ions such as Mn, Zn, Pb, Cu, Cd in water samples showed no interferences on the As (III) determination. The method was applied successfully to real samples collected from arsenic affected areas of West Bengal, India.

Keywords: Adsorptive stripping voltammetry; Arsenic; Impedance; L-cysteine; Surface plasmon resonance.