Derivation of HVR1, HVR2 and HVR3 human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis (PGD) for monogenic disorder

Stem Cell Res. 2016 May;16(3):635-9. doi: 10.1016/j.scr.2016.03.012. Epub 2016 Mar 31.

Abstract

From 106 human blastocyts donate for research after in vitro fertilization (IVF) and preimplantation genetic diagnosis (PGD) for monogenetic disorder, 3 human embryonic stem cells (hESCs) HVR1, HVR2 and HVR3 were successfully derived. HVR1 was assumed to be genetically normal, HVR2 carrying Becker muscular dystrophy and HVR3 Hemophilia B. Despite the translocation t(9;15)(q34.3;q14) detected in HVR2, all the 3 cell lines were characterised in vitro and in vivo as normal hESCs lines and were registered in the Spanish Stem Cell Bank.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastocyst / cytology
  • Cell Differentiation
  • Cells, Cultured
  • Cellular Reprogramming
  • Chromosomes, Human, Pair 15
  • Chromosomes, Human, Pair 9
  • Female
  • Fertilization in Vitro
  • Hemophilia B / diagnosis*
  • Hemophilia B / genetics
  • Human Embryonic Stem Cells / cytology*
  • Human Embryonic Stem Cells / metabolism
  • Human Embryonic Stem Cells / transplantation
  • Humans
  • Karyotype
  • Male
  • Mice
  • Mice, SCID
  • Microscopy, Fluorescence
  • Muscular Dystrophies / diagnosis*
  • Muscular Dystrophies / genetics
  • Preimplantation Diagnosis
  • Teratoma / metabolism
  • Teratoma / pathology
  • Translocation, Genetic