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Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts

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Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts

Joseph C Chen et al. Bio Protoc.

Abstract

Purification and culture of endometrial epithelial cells (eEC) and stromal fibroblasts (eSF) from endometrial biopsies allows for downstream cell-specific in vitro studies. The utility of this protocol is the ease with which cells are purified without contamination from unwanted cell types, and the ability to use patient-paired eEC and eSF in experiments. These methods have been previously published, but here the protocol has been updated for maximum efficiency.

Figures

Figure 1
Figure 1. The endometrial tissue digestion and cell plating procedure
Figure 2
Figure 2. Bright field microscopy of endometrial cell populations
A. Epithelial sheets and fragments; B. Initial attachment of eEC onto Matrigel; C. eEC growing in island-shaped clusters; D. Confluent eEC monolayer with dome-shaped formations; E. eEC culture with eSF overgrowth; F. Confluent eSF. 50× magnification

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