Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts

Bio Protoc. 2015 Oct 20;5(20):e1623. doi: 10.21769/bioprotoc.1623.


Purification and culture of endometrial epithelial cells (eEC) and stromal fibroblasts (eSF) from endometrial biopsies allows for downstream cell-specific in vitro studies. The utility of this protocol is the ease with which cells are purified without contamination from unwanted cell types, and the ability to use patient-paired eEC and eSF in experiments. These methods have been previously published, but here the protocol has been updated for maximum efficiency.