Predation by generalist arthropod predators on Apolygus lucorum (Hemiptera: Miridae): molecular gut-content analysis and field-cage assessment

Abstract

Background: The mirid bug Apolygus lucorum (Meyer-Dür) is a principal pest of cotton that also causes great damage to many other crops in China. A study was conducted to assess the mortality of A. lucorum from generalist arthropod predators using both molecular methods and a field-cage trial. The species-specific primer pair for the detection of A. lucorum tissues in predators was designed according to the sequences of the cytochrome oxidase subunit I (COI) gene.

Results: A total of 2096 generalist predators that consisted of ladybeetles, lacewings and spiders were collected, and A. lucorum remains were detected using the designed primers. Only 1.6% of these predators contained A. lucorum DNA, with the highest positive proportion (6.1%) for Harmonia axyridis larvae. In the field-cage experiment, the daily predation rates of second-instar A. lucorum nymphs by H. axyridis adults and larvae were 4.7 and 5.2% respectively.

Conclusions: The overall low positive proportion of generalist predators with A. lucorum DNA detected using the molecular method, combined with the low predation rate in the field-cage experiment, indicated that the primary generalist predators likely had a limited role in the suppression of A. lucorum in the field. © 2016 Society of Chemical Industry.

Keywords: Miridae; cotton; field-cage trial; generalist predators; species-specific primers.