Broad and Temperature Independent Replication Potential of Filoviruses on Cells Derived From Old and New World Bat Species

J Infect Dis. 2016 Oct 15;214(suppl 3):S297-S302. doi: 10.1093/infdis/jiw199. Epub 2016 Jun 26.


Filoviruses are strongly associated with several species of bats as their natural reservoirs. In this study, we determined the replication potential of all filovirus species: Marburg marburgvirus, Taï Forest ebolavirus, Reston ebolavirus, Sudan ebolavirus, Zaire ebolavirus, and Bundibugyo ebolavirus. Filovirus replication was supported by all cell lines derived from 6 Old and New World bat species: the hammer-headed fruit bat, Buettikofer's epauletted fruit bat, the Egyptian fruit bat, the Jamaican fruit bat, the Mexican free-tailed bat and the big brown bat. In addition, we showed that Marburg virus Angola and Ebola virus Makona-WPGC07 efficiently replicated at 37°C, 37°-41°C, or 41°C, contrary to the hypothesis that temporal elevation in temperature due to flight affects filovirus replication in bats.

Keywords: Ebola virus; Marburg virus; bats; filovirus; replication; temperature.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • Cell Line
  • Chiroptera / virology*
  • Disease Reservoirs / virology*
  • Ebolavirus / immunology
  • Ebolavirus / isolation & purification
  • Ebolavirus / physiology
  • Filoviridae / isolation & purification*
  • Filoviridae / physiology
  • Filoviridae Infections / virology*
  • Hemorrhagic Fever, Ebola / virology*
  • Humans
  • Marburg Virus Disease / virology*
  • Marburgvirus / immunology
  • Marburgvirus / isolation & purification
  • Marburgvirus / physiology
  • Temperature
  • Virus Replication