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. 2016 Jun 29;17(1):142.
doi: 10.1186/s13059-016-1006-0.

AirLab: A Cloud-Based Platform to Manage and Share Antibody-Based Single-Cell Research

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Free PMC article

AirLab: A Cloud-Based Platform to Manage and Share Antibody-Based Single-Cell Research

Raúl Catena et al. Genome Biol. .
Free PMC article

Abstract

Single-cell analysis technologies are essential tools in research and clinical diagnostics. These methods include flow cytometry, mass cytometry, and other microfluidics-based technologies. Most laboratories that employ these methods maintain large repositories of antibodies. These ever-growing collections of antibodies, their multiple conjugates, and the large amounts of data generated in assays using specific antibodies and conditions makes a dedicated software solution necessary. We have developed AirLab, a cloud-based tool with web and mobile interfaces, for the organization of these data. AirLab streamlines the processes of antibody purchase, organization, and storage, antibody panel creation, results logging, and antibody validation data sharing and distribution. Furthermore, AirLab enables inventory of other laboratory stocks, such as primers or clinical samples, through user-controlled customization. Thus, AirLab is a mobile-powered and flexible tool that harnesses the capabilities of mobile tools and cloud-based technology to facilitate inventory and sharing of antibody and sample collections and associated validation data.

Figures

Fig. 1
Fig. 1
a Schematic of the AirLab platform. AirLab is a cloud-based tool with browser and iOS interfaces that powers antibody inventory, panel and experiment design and execution, and experimental data management. b AirLab also enables data sharing within a laboratory or with the community. The database schema summary depicts the main tables and relationships amongst them in the database. Data sharing is user-controlled
Fig. 2
Fig. 2
a Representative listing of antibody clones in the laboratory storage in the iOS tool inferface. Selection of a record uncovers options that can be performed over the record. b Panel building tool enables organization of antibodies per channel and offers basic information and detailed information (shown in iOS interface). c Antibody Panel list showing concentrations selected with calculations performed automatically based on stock concentrations (shown in iOS interface). This facilitates inventory: Those with little volume remaining are marked and those with no sample remaining are archived. Options for sharing and exporting, including as.conf files for control of CyTOF and CyTOF2 instruments, are shown. d Representative listing of antibody clones and associated lots and conjugates (shown in web tool interface). e Antibody Panel list showing concentrations selected with calculations performed automatically based on stock concentrations (shown in web tool interface)

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