Oxidative metabolism of linoleic acid by human leukocytes

Biochem Biophys Res Commun. 1989 Jun 15;161(2):883-91. doi: 10.1016/0006-291x(89)92682-x.


Upon incubation with human leukocytes, [1-14C] linoleic acid is almost exclusively transformed into 13-hydroxy-9Z, 11E-octadecadienoic acid (13-HODE) if the linoleic acid concentration is lower than 50 microM. Identification of 13-HODE was done by GLC-MS at the level of its methyl ester, trimethylsilyl ether and by comparison with authentic 13-HODE in two different HPLC systems. Analysis of the products by chiral phase HPLC shows that 13(S)-hydroxy-9Z, 11E-octadecadienoic acid is by far the major metabolite formed by human leukocytes. Comparison of reactions performed with intact or lyzed cells suggests that the formation of 13(S)-HODE by human leukocytes occurs in two steps, a dioxygenation catalyzed by a 15-lipoxygenase and a reduction of intermediate 13-HPODE by a glutathione-dependent peroxidase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • Humans
  • In Vitro Techniques
  • Kinetics
  • Leukocytes / enzymology
  • Leukocytes / metabolism*
  • Linoleic Acid
  • Linoleic Acids / biosynthesis*
  • Linoleic Acids / metabolism*
  • Oxidation-Reduction


  • Linoleic Acids
  • 13-hydroxy-9,11-octadecadienoic acid
  • Linoleic Acid