Krüpple-like factors 7 and 6a mRNA expression in adult zebrafish central nervous system

Gene Expr Patterns. 2016 May;21(1):41-53. doi: 10.1016/j.gep.2016.06.004. Epub 2016 Jun 27.


Krüpple-like factors (KLFs) are transcription factors with zinc finger DNA binding domains known to play important roles in brain development and central nervous system (CNS) regeneration. There is little information on KLFs expression in adult vertebrate CNS. In this study, we used in situ hybridization to examine Klf7 mRNA (klf7) and Klf6a mRNA (klf6a) expression in adult zebrafish CNS. Both klfs exhibit wide and similar expression in the zebrafish CNS. Brain areas containing strongly labeled cells include the ventricular regions of the dorsomedial telencephalon, the ventromedial telencephalon, periventricular regions of the thalamus and hypothalamus, torus longitudinalis, stratum periventriculare of the optic tectum, granular regions of the cerebellar body and valvula, and superficial layers of the facial and vagal lobes. In the spinal cord, klf7- and klf6a-expressing cells are found in both the dorsal and ventral horns. Numerous sensory structures (e.g. auditory, lateral line, olfactory and visual) and several motor nuclei (e.g. oculomotor, trigeminal, and vagal motor nuclei) contain klf7- and/or klf6a-expressing cells. Our results may provide useful information for determining these Klfs in maintenance and/or function in adult CNS.

Keywords: Auditory nuclei; Brain; Spinal cord; Visual structures; Zinc-finger binding transcription factors.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism
  • Central Nervous System / metabolism*
  • Cerebellum / metabolism
  • Gene Expression Regulation / genetics
  • In Situ Hybridization
  • Nerve Tissue Proteins / biosynthesis*
  • Nerve Tissue Proteins / genetics
  • Neurons / metabolism
  • RNA, Messenger / biosynthesis
  • Spinal Cord / metabolism*
  • Telencephalon / metabolism
  • Thalamus / metabolism
  • Zebrafish / genetics*
  • Zebrafish Proteins / biosynthesis*
  • Zebrafish Proteins / genetics


  • KLF6a protein, zebrafish
  • KLF7a protein, zebrafish
  • Nerve Tissue Proteins
  • RNA, Messenger
  • Zebrafish Proteins