Fungal epidemiology and diversity in cystic fibrosis patients over a 5-year period in a national reference center

Med Mycol. 2016 Nov 1;54(8):781-6. doi: 10.1093/mmy/myw035. Epub 2016 Jun 30.


The knowledge on prevalence rates of yeasts and moulds in patients with cystic fibrosis (CF) in Germany is scarce. The aim of this report is to give an overview of the diversity and epidemiology of fungal species in CF patients. Over a 5-year period, all fungal isolates cultured from microbiological specimen from CF patients were recorded. Beside standard bacteriological culture media two fungal media were used for cultivation. Species were identified by microscopy, biochemical profiling, MALDI-TOF analysis or DNA sequencing methods. In sum, 25,975 clinical samples from CF patients were analyzed. About 75% of CF patients were colonized by yeasts, mainly Candida albicans (38%) and Candida dubliniensis (12%). In 35% of the patients Aspergillus spp. (Aspergillus fumigatus: 29%) were detected, followed by Exophiala dermatitidis and Scedosporium/Lomentospora complex isolates (4% each). Data for other fungal species are shown. Over a 5-year period, the epidemiology of fungal species detected in CF patients was relatively constant. Clinical microbiology laboratories should carefully monitor samples from CF patients for newly occurring fungal pathogens.

Keywords: cystic fibrosis, fungal epidemiology, fungal diversity.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Child
  • Child, Preschool
  • Cystic Fibrosis / complications*
  • Fungi / classification*
  • Fungi / isolation & purification*
  • Germany / epidemiology
  • Humans
  • Infant
  • Infant, Newborn
  • Lung Diseases, Fungal / epidemiology*
  • Lung Diseases, Fungal / microbiology*
  • Male
  • Microbiological Techniques
  • Microscopy
  • Middle Aged
  • Mycological Typing Techniques
  • Prevalence
  • Sequence Analysis, DNA
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Young Adult