Essential Role of the PfRh5/PfRipr/CyRPA Complex during Plasmodium falciparum Invasion of Erythrocytes

Cell Host Microbe. 2016 Jul 13;20(1):60-71. doi: 10.1016/j.chom.2016.06.004. Epub 2016 Jun 30.


Plasmodium falciparum parasites in the merozoite stage invade human erythrocytes and cause malaria. Invasion requires multiple interactions between merozoite ligands and erythrocyte receptors. P. falciparum reticulocyte binding homolog 5 (PfRh5) forms a complex with the PfRh5-interacting protein (PfRipr) and Cysteine-rich protective antigen (CyRPA) and binds erythrocytes via the host receptor basigin. However, the specific role that PfRipr and CyRPA play during invasion is unclear. Using P. falciparum lines conditionally expressing PfRipr and CyRPA, we show that loss of PfRipr or CyRPA function blocks growth due to the inability of merozoites to invade erythrocytes. Super-resolution microscopy revealed that PfRipr, CyRPA, and PfRh5 colocalize at the junction between merozoites and erythrocytes during invasion. PfRipr, CyRPA, and PfRipr/CyRPA/PfRh5-basigin complex is required for triggering the Ca(2+) release and establishing the tight junction. Together, these results establish that the PfRh5/PfRipr/CyRPA complex is essential in the sequential molecular events leading to parasite invasion of human erythrocytes.

MeSH terms

  • Antigens, Protozoan / metabolism*
  • Basigin / metabolism
  • Calcium / metabolism
  • Carrier Proteins / metabolism*
  • Cations, Divalent / metabolism
  • Endocytosis*
  • Erythrocytes / parasitology*
  • Gene Knockdown Techniques
  • Host-Pathogen Interactions
  • Humans
  • Microscopy
  • Models, Biological
  • Plasmodium falciparum / physiology*
  • Protein Binding
  • Protein Multimerization
  • Protozoan Proteins / metabolism*


  • Antigens, Protozoan
  • Carrier Proteins
  • Cations, Divalent
  • CyRPA protein, Plasmodium falciparum
  • Protozoan Proteins
  • RH5 protein, Plasmodium falciparum
  • Ripr protein, Plasmodium falciparum
  • Basigin
  • Calcium