In vivo optical monitoring of transcutaneous delivery of calcium carbonate microcontainers

Elina A Genina; Yulia I Svenskaya; Irina Yu Yanina; Leonid E Dolotov; Nikita A Navolokin; Alexey N Bashkatov; Georgy S Terentyuk; Alla B Bucharskaya; Galina N Maslyakova; Dmitry A Gorin; Valery V Tuchin; Gleb B Sukhorukov

doi:10.1364/BOE.7.002082

In vivo optical monitoring of transcutaneous delivery of calcium carbonate microcontainers

Biomed Opt Express. 2016 May 3;7(6):2082-7. doi: 10.1364/BOE.7.002082. eCollection 2016 Jun 1.

Affiliations

¹ National Research Saratov State University, 83 Astrakhanskaya Street, Saratov 410012, Russia; National Research Tomsk State University, 36 Lenin Avenue, Tomsk 634050, Russia.
² National Research Saratov State University, 83 Astrakhanskaya Street, Saratov 410012, Russia.
³ Saratov State Medical University, 112 Bolshaya Kazachaya Street, Saratov 410012, Russia.
⁴ National Research Saratov State University, 83 Astrakhanskaya Street, Saratov 410012, Russia; Saratov State Medical University, 112 Bolshaya Kazachaya Street, Saratov 410012, Russia.
⁵ National Research Saratov State University, 83 Astrakhanskaya Street, Saratov 410012, Russia; National Research Tomsk State University, 36 Lenin Avenue, Tomsk 634050, Russia; Institute of Precision Mechanics and Control of RAS, 24 Rabochaya Street, Saratov 410028, Russia.
⁶ National Research Saratov State University, 83 Astrakhanskaya Street, Saratov 410012, Russia; School of Engineering and Materials Science, Queen Mary University of London, London, E1 4NS, UK.

Abstract

We have developed a method for delivery of biocompatible CaCO3 microcontainers (4.0 ± 0.8 µm) containing Fe3O4 nanoparticles (14 ± 5 nm) into skin in vivo using fractional laser microablation (FLMA) provided by a pulsed Er:YAG laser system. Six laboratory rats have been used for the microcontainer delivery and weekly monitoring implemented using an optical coherence tomography and a standard histological analysis. The use of FLMA allowed for delivery of the microcontainers to the depth about 300 μm and creation of a depot in dermis. On the seventh day we have observed the dissolving of the microcontainers and the release of nanoparticles into dermis.

Keywords: (110.4500) Optical coherence tomography; (170.0170) Medical optics and biotechnology; (170.0180) Microscopy; (170.1020) Ablation of tissue; (350.4990) Particles.