Mitotic genes are transcriptionally upregulated in the fibroblast irradiated with very low doses of UV-C

Sci Rep. 2016 Jul 5:6:29233. doi: 10.1038/srep29233.

Abstract

Ultraviolet (UV) radiation induces a variety of biological effects, including DNA damage response and cell signaling pathways. We performed transcriptome analysis using microarray in human primary cultured fibroblasts irradiated with UV-C (0.5 or 5 J/m(2)) and harvested at 4 or 12 h following UV exposure. All transcript data were analyzed by comparison with the corresponding results in non-irradiated (control) cells. The number of genes with significantly altered expression (≥2-fold difference relative to the control) is higher in the sample irradiated with high dose of UV, suggesting that gene expression was UV dose-dependent. Pathway analysis on the upregulated genes at 12 h indicates that the expression of some cell cycle-related genes was predominantly induced irrespective of UV-dose. Interestingly, almost all the genes with significant altered expression were cell cycle-related genes designated as 'Mitotic Genes', which function in the spindle assembly checkpoint. Therefore, even a low dose of UV could affect the transcriptional profile.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Proteins / genetics*
  • Cells, Cultured
  • Fibroblasts / physiology*
  • Fibroblasts / radiation effects*
  • Gene Expression Profiling
  • Gene Expression Regulation / radiation effects*
  • Humans
  • Microarray Analysis
  • Mitosis / radiation effects*
  • Ultraviolet Rays*
  • Up-Regulation

Substances

  • Cell Cycle Proteins