Selective mass spectrometric analysis of thiols using charge-tagged disulfides

Analyst. 2016 Oct 7;141(19):5520-6. doi: 10.1039/c6an01210h. Epub 2016 Jul 8.


A simple chemical derivatization technique was developed for electrospray ionization mass spectrometry (ESI-MS) in which thiols and disulfides may be selectively analyzed in a complex matrix and easily characterized. These reagents enhance detection of thiols and disulfides solely due to the nature of the charge-tag derivatization agent and therefore does not require an isotopically labelled substrate. The charged disulfides readily and exclusively react with thiols in a complex matrix in a short amount of time. Furthermore, the synthesis of these reagents is simple and results in a highly pure and stable reagent. The efficacy of this reaction was demonstrated using on-line monitoring, while the scope and usefulness of the reaction was demonstrated in petroleum fractions.