Cell signaling regulation by protein phosphorylation: a multivariate, heterogeneous, and context-dependent process

Curr Opin Biotechnol. 2016 Aug:40:185-192. doi: 10.1016/j.copbio.2016.06.005. Epub 2016 Jul 6.


Proper spatiotemporal regulation of protein phosphorylation in cells and tissues is required for normal development and homeostasis, but aberrant protein phosphorylation regulation leads to various diseases. The study of signaling regulation by protein phosphorylation is complicated in part by the sheer scope of the kinome and phosphoproteome, dependence of signaling protein functionality on cellular localization, and the complex multivariate relationships that exist between protein phosphorylation dynamics and the cellular phenotypes they control. Additional complexities arise from the ability of microenvironmental factors to influence phosphorylation-dependent signaling and from the tendency for some signaling processes to occur heterogeneously among cells. These considerations should be taken into account when measuring cell signaling regulation by protein phosphorylation.

Publication types

  • Review

MeSH terms

  • Animals
  • Cell Physiological Phenomena*
  • Humans
  • Phosphoproteins / metabolism*
  • Phosphorylation
  • Signal Transduction*


  • Phosphoproteins