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, 48 (4), 283-96

Histological Study on the Protective Role of Vitamin B Complex on the Cerebellum of Diabetic Rat

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Histological Study on the Protective Role of Vitamin B Complex on the Cerebellum of Diabetic Rat

Sohair A Eltony. Tissue Cell.

Abstract

Background: Disorder in cerebellar structure was reported in diabetes mellitus. B vitamins are involved in many significant metabolic processes within the brain.

Aim of the work: To detect the protective role of vitamin B complex on the histological structure of the cerebellum of experimentally induced diabetic rat.

Material & methods: Eighteen adult male Wistar rats were divided into two groups. Group I: normal vehicle control (n=6). Group II: streptozotocin-induced diabetic rats (n=12), which was equally divided into two subgroups; IIA (diabetic vehicle control), IIB (diabetic vitamin B complex-treated): streptozotocin-induced diabetic rats treated with vitamin B complex (1mg/kg/day) for 6 weeks. Specimens from the cerebellum were processed for light and electron microscopy.

Results: In vitamin B complex treated group, the histological changes in Purkinje cells, astrocytes and oligodendrocytes were improved compared with the diabetic non-treated group. The white matter revealed intact myelinated axons. Inducible nitric oxide synthase (iNOS) and caspase-3 expression reduced. Glial fibrillary acidic protein (GFAP) expression revealed less activated astroglia. The number of Purkinje cells/mm(2) significantly increased. While, the number of GFAP positive astrocytes/mm(2) significantly decreased. In addition, the blood glucose level was reduced.

Conclusion: Vitamin B complex protected the cerebellum from the histological changes which occurred in STZ- induced diabetic rats.

Keywords: Diabetic encephalopathy; Gliosis; Vitamin B complex.

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