Fine specificity of the antibody response to Epstein-Barr nuclear antigen-2 and other Epstein-Barr virus proteins in patients with clinically isolated syndrome: A peptide microarray-based case-control study

J Neuroimmunol. 2016 Aug 15;297:56-62. doi: 10.1016/j.jneuroim.2016.05.012. Epub 2016 May 13.


We analyzed the fine specificity of antibodies to Epstein-Barr nuclear antigen-2 (EBNA-2) and other Epstein-Barr virus (EBV) proteins in 29 patients with clinically isolated syndrome (CIS, the first clinical manifestation of multiple sclerosis [MS]) and 29 controls with a peptide microarray containing 117 overlapping peptides representing the full-length EBNA-2 protein and 71 peptides from 8 further EBV proteins. While EBV peptide antibodies were elevated in CIS, suggesting that EBV contributes to MS early during disease development, they discriminated groups only slightly better than EBNA-1 antibodies. Thus, the additional value of EBV peptide antibodies as diagnostic biomarkers for CIS appears moderate.

Keywords: Antibodies; Clinically isolated syndrome; Epstein-Barr nuclear antigen; Epstein-Barr virus; Multiple sclerosis; Peptide microarray.

MeSH terms

  • Adult
  • Animals
  • Antibodies, Viral / metabolism*
  • Antibody Formation / physiology*
  • Disability Evaluation
  • Enzyme-Linked Immunosorbent Assay
  • Epstein-Barr Virus Infections / complications*
  • Epstein-Barr Virus Infections / immunology
  • Epstein-Barr Virus Nuclear Antigens / immunology*
  • Female
  • Herpesvirus 4, Human / immunology
  • Herpesvirus 4, Human / pathogenicity
  • Humans
  • Immunoglobulin G / blood
  • Magnetic Resonance Imaging
  • Male
  • Mice, Inbred BALB C
  • Middle Aged
  • Multiple Sclerosis / blood
  • Multiple Sclerosis / diagnostic imaging
  • Multiple Sclerosis / etiology*
  • Multiple Sclerosis / immunology
  • Protein Array Analysis
  • Statistics, Nonparametric
  • Viral Proteins / immunology*
  • Young Adult


  • Antibodies, Viral
  • EBNA-2 protein, Human herpesvirus 4
  • Epstein-Barr Virus Nuclear Antigens
  • Immunoglobulin G
  • Viral Proteins
  • EBV-encoded nuclear antigen 1