Mapping the transition state and pathway of protein folding by protein engineering

Nature. 1989 Jul 13;340(6229):122-6. doi: 10.1038/340122a0.


In the transition state for unfolding of barnase, the hydrophobic core between the major alpha-helix and beta-sheet is somewhat weakened, the C terminus of the major helix is largely intact but its N terminus is exposed and a major loop has been invaded by solvent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacillus / enzymology
  • Bacterial Proteins
  • Kinetics
  • Mutation
  • Peptide Mapping* / methods
  • Protein Conformation
  • Protein Denaturation
  • Protein Engineering* / methods
  • Ribonucleases / genetics
  • Ribonucleases / isolation & purification*
  • Thermodynamics


  • Bacterial Proteins
  • Ribonucleases
  • Bacillus amyloliquefaciens ribonuclease