N-acetylcysteine pretreatment attenuates paraquat-induced lung leak in rats

B M Hybertson; A S Lampey; J H Clarke; Y Koh; J E Repine

doi:10.1080/13510002.1995.11747008

N-acetylcysteine pretreatment attenuates paraquat-induced lung leak in rats

Redox Rep. 1995 Nov;1(5):337-42. doi: 10.1080/13510002.1995.11747008.

Authors

B M Hybertson¹, A S Lampey¹, J H Clarke¹, Y Koh¹, J E Repine¹

Affiliation

¹ a Webb-Waring Institute for Biomedical Research and the Department of Medicine , University of Colorado Health Sciences Center , Denver , Colorado and the Department of Internal Medicine, Asan Medical Center , University of Ulsan School of Medicine , Seoul , Korea.

PMID: 27405831
DOI: 10.1080/13510002.1995.11747008

Abstract

We investigated the effects of N-acetylcysteine (NAC) pretreatment on paraquat-induced lung inflammation and leak. We found that administering a single intravenous dose (60 mg/kg) of paraquat rapidly (2 h) increased lung leak, lung lavage cytokine-induced neutrophil chemoattractant (CINC) levels, and lung myeloperoxidase (MPO) activity in rats. Rats pretreated with NAC (150 mg/kg, intraperitoneally) had increased lung tissue glutathione (GSH + GSSG) levels compared to saline-pretreated rats. In addition, rats pretreated with NAC and then given paraquat 2.5 h later had decreased lung leak compared to saline-pretreated rats given paraquat. In contrast, NAC pretreated rats given paraquat had the same lung lavage CINC levels and lung tissue MPO activity as saline-pretreated rats given paraquat. Our results indicate that paraquat causes an oxidative injury which may be decreased by the GSH-increasing or other properties of NAC.