Expression of metalloproteinases (MMP-2 and MMP-9) in basal-cell carcinoma

Abstract

The aim of this study was to compare the expressions of mRNA for metalloproteinases (MMP-2 and MMP-9) and type IV collagen in two different histological types of basal-cell carcinoma (BCCs; nodular and infiltrative) and in normal tissues from the tumor interface. The study included biopsy specimens of the skin involved with BCC and normal skin adjacent the lesion. The expressions of mRNA for MMP-2, MMP-9 and type IV collagen were determined by means of RT-PCR (Reverse transcription polymerase chain reaction). The level of type IV collagen mRNA in nodular and infiltrative BCCs turned out to be significantly lower, and the expressions of MMP-2 and MMP-9 mRNA significantly higher than in normal tissues adjacent to these tumors. The expression of mRNA for MMP-9 but not for MMP-2 was significantly higher in infiltrative BCCs than in the nodular BCCs. In turn, normal tissues adjacent to nodular BCCs showed significantly higher levels of mRNA for MMP-2 and significantly lower levels of type IV collagen mRNA than the normal tissues from the interface of infiltrative BCCs. The findings suggest that MMP-2 and MMP-9 could be used as prognostic factors of BCCs.

Keywords: Basal-cell carcinoma; Invasiveness; Matrix metalloproteinases; Molecular diagnosis; Resection margin; Type IV collagen.

Fig. 1

Mean (±SD) expressions of mRNA for type IV collagen in the tissues (T) of nodular (nBCC) and infiltrative (iBCC) basal-cell carcinoma and in normal tissues adjacent to the tumors (NT)

Fig. 2

Mean (±SD) expressions of mRNA for matrix metalloproteinases 2 (MMP2) and 9 (MMP9) in the tissues (T) of nodular (nBCC) and infiltrative (iBCC) basal-cell carcinoma and in normal tissues adjacent to the tumors (NT)